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KMID : 0363320090300010212
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Journal of Korean Oriental Internal Medicine
2009 Volume.30 No. 1 p.212 ~ p.227
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Effects of Patriniae Radix Extract on The Membranous Nephropathy Induced by Cationic Bovine Serum Albumin in Mice
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Chang Sun-Kyu
Cho Chung-Sik
Kim Cheol-Jung
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Abstract
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Objective: Membranous nephropathy (MN) is one of the most commonest forms of glomerular disease in man and the most frequent cause of the adult idiopathic nephrotic syndrome. Some investigators recommend no treatment, while others propose aggressive therapy, including prednisolone plus an immunosupressant such as chlorambucil or cyclophosphamide. But a more effective way to treat MN is not defined yet. This study was to evaluate the effects of Patriniae Radix extract (PRE) on the MN induced by cBSA in mice.
Methods: Mice were divided into 4 groups. The first group (normal) was injected with saline. The second group (control) was treated with cBSA (10 mg/kg i.p) only. The third group, named PRE-2S0, was treated with cBSA (10 mg/kg i.p) and PRE (250 mg/kg, p.o). The fourth group, PRE-500, was treated with cBSA (10mg/kg i.p) and PRE (500mg/kg, p.o). After cBSA and PRE treatment for 4 weeks, we measured change of body weight, 24hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN, creatinine, IgG, IgA, IgM, TNF-α, IL-1β, and IFN-γ levels and the mRNA expression of IFN-γ, IL-6, and IL-10. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope.
Results: The levels of 24 hrs proteinuria and serum triglyceride. BUN. IgG. TNF-α, and IL-1β significantly decreased in both PRE groups, while the level of serum albumin significantly increased in both PRE groups. The mRNA expression of IL-10 in splenocytes considerably increased in both PRE groups. The mRNA expression of IFN-γ and IL-6 in splenocytes considerably increased in both PRE group. In histological findings of kidney tissue, thickening of GBM decreased in both PRE groups.
Conclusions: The present study suggests that PRE is effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.
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KEYWORD
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Patriniae Radix Extract(PRE), membranous nephropathy(MN), cationic bovine serum albumin(cBSA)
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